Identification of Syn-copalyl Diphosphate Synthase
نویسندگان
چکیده
The present invention relates to the isolation, puri?cation, sequencing, and functional characterization of the class I and II terpene sequences OsCPSlem, OsCPS2em, OsCPS4Syn, and OsDTS2. Transcriptional control of OsCPSlem provides a means of regulating production of gibberellin phytohormone, While transcriptional control of OsCPS2em, OsDTS2, and OsCPS4Syn provides a means of regulating defensive phy tochemical biosynthesis. Further, these enzymatic genes offers a means by Which terpenoid production may be more generally modulated, speci?cally including genetic engineer ing, as Well as ex vivo uses. Disciplines Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Natural Products Chemistry and Pharmacognosy This patent is available at Iowa State University Digital Repository: http://lib.dr.iastate.edu/patents/284 (12) United States Patent Peters et al. US007525016B1 US 7,525,016 B1 Apr. 28, 2009 (10) Patent N0.: (45) Date of Patent: (54) IDENTIFICATION OF SYN-COPALYL DIPHOSPHATE SYNTHASE (75) Inventors: Reuben J. Peters, Ames, IA (US); Meimei Xu, Ames, IA (US) (73) Assignee: Iowa State University Research Foundation, Inc., Ames, IA (US) ( * ) Notice: Subject to any disclaimer, the term of this patent is extended or adjusted under 35 U.S.C. 154(b) by 261 days. (21) Appl.No.: 11/135,267 (22) Filed: May 23, 2005 Related US. Application Data (60) Provisional application No. 60/573,472, ?led on May 21, 2004. (51) Int. Cl. C12N 15/29 C12N 15/52 C12N 15/82 (2006.01) A01H 5/00 (2006.01) (52) US. Cl. ..................... .. 800/298; 800/278; 800/279; 435/69.1; 435/468 (58) Field of Classi?cation Search ..................... .. None See application ?le for complete search history. (2006.01) (2006.01) (56) References Cited U.S. PATENT DOCUMENTS 2007/0141574 A1* 6/2007 Keasling etal. .............. .. 435/6
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Product Rearrangement from Altering a Single Residue in the Rice syn-Copalyl Diphosphate Synthase
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